Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLNLIYNEWLKIFSRAGTWVMIGILGLTMVGFAFLANHFSAGESNSHWKQELQAQNAELKKEIKEDPSLKDGYKETITLNDYRIEHNIPSDTGYTVWSYVTDSANFTILTGLFTIIIAAGIVANEFNWGTIKLLMIRPLSRFQILMSKYITVLLFGLLLLLILFIGSTLLGLIFFGTGGETAANIHLIYKDGHVIEQNMMGHLATTYLSESVSALMVATMAFMLSAVFRNSSLAVGFSIFLLVAGTTATAFIAAKFDWAKYILFANVDLTQYVDGTPLIKGMTMTFSLVMLAIYFIIFLLLAFGIFMKRDIAN 2M04 Chain:B ((1-25)) --------------------------------------------EEQWAREIGAQLRRMADDLNAQYER----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2M04.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 9 844 93.72 33.74 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain B : 0.38 3D Compatibility (PKB) : 93.72 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.38 QMean score : 0.805

(partial model without unconserved sides chains): PDB file : Tito_2M04.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2M04-query.scw PDB file : Tito_Scwrl_2M04.pdb: