Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLKELFVNLTILITFNYLFTHLFKERLVHKKDSISFQAVKGLACGLLGVILMVFGFTYQHSIIDLRNIPIMIAALYGGWVSTATALAMITAGRLLITMNTSALYSVIIICIAAIPSLIVSRRKKVQLKHAFYLLIITNSLISFSFYFLIDLHSYELHLYFWIISIAGGMLSLYIIDHETNAHLLFKQYKFQAHFDFLTGVYNRRKFEETTKALYQQAADTPHFQFALIYMDIDHFKTINDQYGHHEGDQVLKELGLRLKQTIRNTDPAARIGGEEFAVLLPN-----CSLDKAARIAERIRSTVSDAPIVLTNGDELSVTISLGAAHYPNNTEQPGSLPILADQMLYKAKETGRNRVCFSEKKE 4RNF Chain:A ((2-166)) -----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AYYDALTHLPNRTLFQDRLHTALQQAERNGQW-VVLMFLDLD-----NDSLGHAAGDRMLQEVATRLSACVSQDDTVARMGGDEFTLLLPSQGDREIALKRAIQVAELILGRLA-RPFTL-EGREFFVTASIGVALSPQDGAELSLLMKNADTAMYHAKEMGKNNFQF-----

General information: TITO was launched using: RESULT: Template: 4RNF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 731 -98758 -135.10 -637.15 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : -135.10 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.533

(partial model without unconserved sides chains): PDB file : Tito_4RNF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4RNF-query.scw PDB file : Tito_Scwrl_4RNF.pdb: