Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MENQEVVLSIDAIQEPEQIKFNMSLKNQSERAIEFQFSTGQKFELVVYDSEHKERYRYSKEKMFTQAFQNLTLESGETYDFSDVWKE------VPEPGTYEVKVTFKGRAENLKQVQAVQQFEVK 4ZZF Chain:A ((168-223)) ------------------------------------------PAIQILNENNELVKTIPLKDYN--------GQKGY---INFEWDGTNEKGEKVPKGNYKIKAEYNLD----------------

General information: TITO was launched using: RESULT: Template: 4ZZF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 126 -11289 -89.60 -225.78 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -89.60 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.561

(partial model without unconserved sides chains): PDB file : Tito_4ZZF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZZF-query.scw PDB file : Tito_Scwrl_4ZZF.pdb: