Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLYMSVDMEGISGLPDDTFVDSGKRNYERGRLIMTEEANYCIAEAFNSGCTEVLVNDSHSKMNNLMVEKLHPEADLISGDVKPFSMVEGLDDTFRGALFLGYHARASTPGVMSHSMIFGVRHFYINDRPVGELGLNAYVAGYYDVPVLMVAGDDRAAKEAEELIPNVTTAAVKQTISRSAVKCLSPAKAGRLLTEKTAFALQNKDKVKPLTPPDRPVLSIEFANYGQAEWANLMPGTEI-KTGTTTVQFQAKDMLEAYQAMLVMTELAMRTSFC 1HI9 Chain:A ((1-274)) MKLYMSVDMEGISGLPDDTFVDSGKRNYERGRLIMTEEANYCIAEAFNSGCTEVLVNDSHSKMNNLMVEKLHPEADLISGDVKPFSMVEGLDDTFRGALFLGYHARASTPGVMSHSMIFGVRHFYINDRPVGELGLNAYVAGYYDVPVLMVAGDDRAAKEAEELIPNVTTAAVKQTISRSAVKCLSPAKRGRLLTEKTAFALQNKDKVKPLTPPDRPVLSIEFANYGQAEWANLMPGTEIKT-GTTTVQFQAKDMLEAYQAMLVMTELAMRTSFC

General information: TITO was launched using: RESULT: Template: 1HI9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1728 -164200 -95.02 -601.47 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.99 3D Compatibility (PKB) : -95.02 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.99 QMean score : 0.767

(partial model without unconserved sides chains): PDB file : Tito_1HI9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1HI9-query.scw PDB file : Tito_Scwrl_1HI9.pdb: