Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSELLATYLLT-EPGADTEKKAEQIATGLTVGSWTDLPLVKQEQMQKHKGRVIKVEEREGTAASEKQAVITIAYP-----EINFSQDIPALLTTVFGKLSLDGKIKLIDLHFSEAFKRALPGPKFGVYGIRKLLGEFERPLLMSIFKGVIGRDLSDIKEQLRQQALGGVDLIKDDEIFFETGLAPFETRIAEGKQILKETYEQTGHKTLYAVNLT-GRTADLKDKARRAAELGADALLFNVFAYGLDVMQGLAE---DPEIPVPIMAHPAVSGAFTSSPFYGFSHALLLGKLNRYCGADFSLFPSPYGSVALPRADALAIHEECVREDA----------FNQTFA-------VPSAGIHPGMVPLLMRDFGIDHIINAGGGVHGHPNGAQGGGRAFRAIIDAVLEAQPIDEKAEQCKDLKLALDKWGKAEAV 1RBO Chain:L ((34-431)) -TDILAAFRVSPQPGVPPEEAGAAVAAESSTGTWTTVWTDGLTNLDRYKGRCYHIEP---VAGEENQYICYVAYPLDLFEEGSVTNMFTSIVGNVFGFKALRA-LRLEDLRIPVAYVKTFQGPPHGIQVERDKLNKYGRPLLGCTIKPKLGLSAKNYGRAVYECLRGGLDFTKDDENVNSQPFMRWRDRFLFCAEALYKAQAETGEIKGHYLNATAGTCEDMMKRAVFARELGVPIVMHDYLTGGFTANTTLSHYCRDNGLLLHI--HRAMHAVIDRQKNHGM-HFRVLAKALRLSGGDHIHSGTVVGKLEGERDITLGF-VDLLRDDYTEKDRSRGIYFTQSWVSTPGVLPVASGGIHVWHMPALTEIFGDDSVLQFGGGTLGHPWGNAPGAVANRVALEACVQAR-------------------------

General information: TITO was launched using: RESULT: Template: 1RBO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain L - contact count / total energy / energy per contact / energy per residue : 2014 -197919 -98.27 -533.47 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain L : 0.72 3D Compatibility (PKB) : -98.27 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.501

(partial model without unconserved sides chains): PDB file : Tito_1RBO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1RBO-query.scw PDB file : Tito_Scwrl_1RBO.pdb: