Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MADSFLFYNLSEAQMTFQDVMERLKAFVQKDPRSSYVLSIGTDSQVYRDYTKFI-TALHLHRTGKGAWGCLKNHTVDRPIHSLREK---ISLETAYSQETAAHIL-DGHLMDITDLLLPFTGEGADLTFEVHLDI-GKKGLTKDLIQEMTGRITSMGIEAKIKPDSYTAFSYANRFTK 2E3U Chain:A ((39-164)) -----------------------------KIPKDRIAVLIGKKGQTKKEIEKRTKTKITIDSETGEVWITSTKETED-PLAVWKARDIVLAIGRGFSPERAFRLLNEGEYLEIINLTDII------ALPRVRGRIIGRKGRTRQIIEEMSGASVSV----------------------

General information: TITO was launched using: RESULT: Template: 2E3U.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 361 -30686 -85.00 -269.18 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -85.00 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.146

(partial model without unconserved sides chains): PDB file : Tito_2E3U.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2E3U-query.scw PDB file : Tito_Scwrl_2E3U.pdb: