Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKMMDANEIISFIQNSTKSTPVKVYVKGELEGINFGESAKAFINGNTGVVFGEWSEIQTAIEENQSKIEDYVVENDRRNSAIPMLDLKNIKARIEPGAIIRDQVEIGDNAVIMMGASINIGSVIGEGTMIDMNVVLGGRATVGKNCHIGAGSVLAGVIEPPSAKPVVIEDDVVIGANAVVLEGVTVGKGAVVAAGAIVVNDVEPYTVVAGTPAKKIKDIDEKTKGKTEIKQELRQL 4EA7 Chain:A ((102-214)) ---------------------------------------------------------------------------------------------IHPSAVVSPSVRLGEGVAVMAGVAINADSWIGDLAIINTGAVVDHDCRLGAACHLGPASALAGGVS--------VGERAFLGVGARVIPGVTIGADTIVGAGGVVVRDLPDSVLAIGVPAK----------------------

General information: TITO was launched using: RESULT: Template: 4EA7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 695 -136664 -196.64 -1209.42 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -196.64 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.572

(partial model without unconserved sides chains): PDB file : Tito_4EA7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4EA7-query.scw PDB file : Tito_Scwrl_4EA7.pdb: