Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFQSTEIGIDLGTANILVYSKNKG---IILNE------PSVVAVDTTTKAVLAIGA-------------DAKNMIGKT-------------PGKIV--AVRPM--------KDGVIADYDMTTDLLKHIMKKAAKSIGMSFRKPNVVVCTPSGSTAVERRAISDAVKNCGAKNVHLIEEPVAAAIGADLPVDEPVANVVV-DIGGGTTEVAIIS-----FGGVVSCHSIRIGGDQLDEDIVSFVRKKYNLLIGE------RTAEQVKMEI---GHALIEHIPEAMEI----RGRDLVTGLPKTIMLQSNEIQDAMRESLLHILEAIRATLEDCPPELSGDIVDRGVILTGGGALLNGIKEWLTEEIVVPVHVAQ--NPLESVAIGTGRSLEVIDKLQKAIK 3LDL Chain:A ((6-374)) ----TVVGIDLGTTYSCVGVFKNGRVEIIANDQGNRITPSYVAFTPEGERLIGDAAKNQLTSNPENTVFDAKRLIGRTWNDPSVQQDIKFLPFKVVEKKTKPYIQVDIGGGQTKTFAPEEISAMVLTKMKETAEAYLGK--KVTHAVVTVPAYFNDAQRQATKDAGTIAGLNVMRIINEPTAAAIAYGLDKREGEKNILVFDLGGGTFDVSLLTIDNGVFEVVATNGDTHLGGEDFDQRVMEHFIKLYKKKTGKDVRKDNRAVQKLRREVEKAKRALSSQHQARIEIESFYEGEDFSETLTRAKFEELN--MDLFRST----MKPVQKVLEDS--DLKKSDIDE-IVLVGGSTRIPKIQQ-LVKEFFNGKEPSRGINPDEAVAYG----------------

General information: TITO was launched using: RESULT: Template: 3LDL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1535 -129591 -84.42 -427.69 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -84.42 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.443

(partial model without unconserved sides chains): PDB file : Tito_3LDL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3LDL-query.scw PDB file : Tito_Scwrl_3LDL.pdb: