Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MITYVFPGQGSQKQGMGSGLFDEF---KELTDQADEILGYSIKRLCLENPYSNLNKTQFTQPALYVVNALSYLKKIRDEEVKPDFVAGHSLGEYNALFAAEAFDFETGLQLVRKRGELMSL---ISNGGMAAVMGLNEEQVAKALKEYHLHD-VDIANVNAPYQIVISGKKDEIEKAASLFETMTEVTMVLPLNVSGAFHSRYMNKAKEEFEEFLHAFYFSPPSIPVISNVYAKPYTYEF-MKQTLADQINHSVKWTDSISYLMKKGHMEFEEVGPGNVLTGLIHRIKKDAEAMPR 3HJV Chain:A ((12-300)) ----VFPGQGSQAVGMLADLAEQYAVVKQTFAEASEVLGYDLWALVQDGPVEDLNQTFRTQPALLAASVAIWRVWQQLGLEQPAVLAGHSLGEYSALVCAGVIDFKQAIKLVELRGQLMQQAVPAGTGAMYAIIGLEDEAIAKACADAAQGEVVSPVNFNSPGQVVIAGQKDAVERAGVLCKEAG-AKRALPLPVSVPSHCALMKPAADELAKTLAELEFNAPQIPVINNVDVVAETDPVKIKDALIRQLYSPVRWTECVEQMSAQGVEKLIEMGPGKVLTGLTKRIVKTLEGV--

General information: TITO was launched using: RESULT: Template: 3HJV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1562 -169568 -108.56 -603.44 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -108.56 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.524

(partial model without unconserved sides chains): PDB file : Tito_3HJV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3HJV-query.scw PDB file : Tito_Scwrl_3HJV.pdb: