Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---------------------------------MERHYYTYLIKEEFANHYFGRESVMFELFQDYHWT-SLEKQQYEMTEKQIQYITQP----IPILHMHQRLKMNLNKTDYRQLDYIYRIALPKA---KGHATFMMKEHMIEIVASGDYEAETIFFEVLRKVSPCFLAM----------------DFNSKRYGWLNPVKERNFV 4U9V Chain:B ((1-197)) SVCAKVDAANRLGDPLEAFPVFKKYDRNGLNVSIECKRVSGLEPATVDWAFDLTKTNMQTMYEQSEWGWKDREKREEMTDDRAWYLIAWENSSVPVAFSHFRFDVECG----DEVLYCYEVQLESKVRRKGLGKFLIQ--ILQLMANST-QMKKVMLTVFKHNHGAYQFFREALQFEIDDSSPSMSGCCGEDCSYEILSRRTKF-

General information: TITO was launched using: RESULT: Template: 4U9V.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 555 23441 42.24 167.44 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain B : 0.68 3D Compatibility (PKB) : 42.24 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.142

(partial model without unconserved sides chains): PDB file : Tito_4U9V.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4U9V-query.scw PDB file : Tito_Scwrl_4U9V.pdb: