Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNELKDFFFLGKPIQTEIGEIDFIRLKDYPLYTKELSMLRMNKKSLIKEYSRFNEDGSLDPFIIEMKKRDLYEIVHSVL-PDFHEAYFKVFS---------KVLINKDSLSLIGKHNFPRLRKLILDMHCITEDK------VVDNDELQEFHDISKSLKQQ-------DSQSDLKDIVSCVAA----FNGYTYEEISEMTMYQLY---LSFYRMAEVMNYNTTTLFATVSPDVKVSDWSSHINLYKEESYHLSTKDAKNIEQLFGG 4ART Chain:A ((22-270)) IDATNDEEKLADIVENEI-EKEIRKIENF-----YYYILRDGKIYPASDYDIEVEKGKRS-------ANDIYAFVETDVTRDFDEFLFDIDYGLPSISDILKFYLEKAGFRIANEVPTPNLK---YYIHAVVEFPQYLAVNIYDIDSLARALRIPQIVEQKLGNKPRTITADEFNDIERIVAEEQPILAGYTYDEALRIPYHYYVDHNNSFKDDALKIAHAYLQLFPT-----------PYQVCYEWKARWFNKIDCLKLERLK--

General information: TITO was launched using: RESULT: Template: 4ART.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 894 -26368 -29.49 -127.38 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -29.49 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.256

(partial model without unconserved sides chains): PDB file : Tito_4ART.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ART-query.scw PDB file : Tito_Scwrl_4ART.pdb: