Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------------------------------------------------------MEKLFKEVK--LEELENQKGSGLGKAQCAALWLQCASGGTIGCGGGAVACQNYRQFCR---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- 1KBL Chain:A ((1-375)) AKWVYKFEEGNASMRNLLGGKGCNLAEMTILGMPIPQGFTVTTEACTEYYNSGKQITQEIQDQIFEAITWLEELNGKKFGDTE-DPLLVSVRSGARASMPGMMDTILNLGLNDVAVEGFAKKTGNPRFAYDSYRRFIQMYSDVVMEVPKSHFEKIIDAMKEEKGVHFDTDLTADDLKELAEKFKAVYKEAMNGEEFPQEPKDQLMGAVKAVFRSWDNPRAIVYRRMNDIPGDWGTAVNVQTMVFGNKGETSGTGVAFTRNPSTGEKGIYGEYLINAQGEDVVAGVRTPQPITQLENDMPDCYKQFMDLAMKLEKHFRDMQDMEFTIEEGKLYFLQTRNGKRTAPAALQIACDLVDEGMITEEEAVVRIEAKSLDQL

General information: TITO was launched using: RESULT: Template: 1KBL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 167 14697 88.00 267.21 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain A : 0.55 3D Compatibility (PKB) : 88.00 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.55 QMean score : 0.031

(partial model without unconserved sides chains): PDB file : Tito_1KBL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1KBL-query.scw PDB file : Tito_Scwrl_1KBL.pdb: