Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTLHVQTASSSYPVFIGQGIRKKACELLTSLNRPLTRIMFVTDEEVDRLYGDEMLHLLQEKW----PVKKVTV---PSGEQAKSMDMYTKLQSEAIRFH--MDRSSCIIAFGGGVVGDLAGFVAATFMRGIDFIQMPTTLLAH-DSAVGGKVAVNHPLGKNLIGAFYQPKAVLYDTDFLRSLPEKELRSGMAEVIKHAFIYDR---AFLEELLNIHSL-----RDITNDQ---------LNDMIFKGISIKASVVQQDEKEEGIRAYLNFGHTLGHAVEAEYGYGQITHGDAVALGM----QFALYIS-EKTVGCEMDRKRLVSWLKSLGYPSQIR----------KETETSVLLNRMMNDKKTRGGKIQFIVLNELGKVADHTFSRNELESWLNKWRLEETS 1SG6 Chain:A ((37-372)) ------------------------------------TTYVLVTDTNIGSIYTPSFEEAFRKRAAEITPSPRLLIYNRPPGEVSKSRQTKADIEDWMLSQNPPCGRDTVVIALGGGVIGDLTGFVASTYMRGVRYVQVPTTLLAMVDSSIGGKTAIDTPLGKNLIGAIWQPTKIYIDLEFLETLPVREFINGMAEVIKTAAISSEEEFTALEE--NAETILKAVRREVTPGEHRFEGTEEILKARILASARHKAYVVSA-----GLRNLLNWGHSIGHAIEAIL-TPQILHGECVAIGMVKEAELARHLGILKGVAV----SRIVKCLAAYGLPTSLKDARIRKLTAGKHCSVDQLMFNMALDKK-------IVLLSAIG-------------------------

General information: TITO was launched using: RESULT: Template: 1SG6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1366 -19473 -14.26 -69.05 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -14.26 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.539

(partial model without unconserved sides chains): PDB file : Tito_1SG6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1SG6-query.scw PDB file : Tito_Scwrl_1SG6.pdb: