Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---------------MNKTDKLLESLNVFIQKAEENQYKQLGEMVPDFPGKSNIPKYVEEYEKGIA----RLLRRQHKKFLDGLKGFVSKDSEETLKALLVFFTQNL--FAEDDFEEEFQELT------EGFLQQTIE---EMAEVIMDSIDPEV----PFKVLSNRTINWIKDWSKKLAEIMKLNTHEVVEDVLTNAIENGSSIQDIELTLKDMPQFDRERARTTAITEVLAASSAAQHESYAQSPAVKKKKWRHSGGKKNNPRENHIDLDGTVIGVDE---EFQIPGSSETCMFPRDPKLSTRERVNCHCVLS----PVVDSKILGLSPEEKEEIQREVLANME------ 1TXD Chain:A ((766-1138)) PPNWQQLVSREVLLGLKPCEIKRQEVINELFYTERAHVRTLKVLDQVFYQRVSREGILSPSELRKIFSNLEDILQLHIGLNEQMKAVRKRNETSVIDQIGEDLLTWFSGPGEEKLKHAAATFCSNQPFALEMIKSRQKKDSRFQTFVQDAESNPLCRRLQLKDIIPTQMQRLTKYPLLLDNIAKYTEWPTEREKVKKAADHCRQILNFVNQAVKEAENKQRLEDYQRRLDTSSLVEELRNLDLTKRKMIHEGPLVWKVNRDKTIDLYTLLLEDILVLLQKQDDRLVLRCTFSPVIKLSTVLVRQVATNKALFVISMSDNGAQIYELVAQTVSEKTVWQDLICRMAASVKEQS

General information: TITO was launched using: RESULT: Template: 1TXD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1180 83646 70.89 274.25 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : 70.89 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.360

(partial model without unconserved sides chains): PDB file : Tito_1TXD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1TXD-query.scw PDB file : Tito_Scwrl_1TXD.pdb: