Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYDTIVIGAGQAGISIGYYLKQSD--QKFIILDKSHEVGESWKDRYDSLVLFTSRMYSSLPGMHLEGEKHGFPSKNEIVAYLKKYVKKFEIPIQLRTEVISVLKIKNYFLIKT----NREEYQTKNLVIATGPFHTPNIPSISKDLSDNINQLHSSQ-YKNSK----QLAYGNVLVVGGGNSGAQIAVELSK-ERVTYLACSNKLVYFPLMIGKRSIFWWFDKLGVLHASHTSIVGKFIQKKGDPVFGHELKHAIKQKEIILK--KRVIAAKQN---EIIFKDSSTLEVNNIIWATGFRNPLCWINIKGVLDQEGRIIHH-RGVSPVEGLYFIGLPWQHKR-------GSALLQGVGNDAEYIVKQMNGE 1NHP Chain:A ((1-315)) -MKVIVLGSSHGGYEAVEELLNLHPDAEIQWYEKGDFISFLSA-G---M----Q---LYLEG--------KVKDVNSVRYMTGEKMESRGVNVFSNTEITAIQPKEHQVTVKDLVSGEERVENYDKLIISPGA--VPFELDIPGKDLDNIYLMRGRQWAIKLKQKTVDPEVNNVVVIGSGYIGIEAAEAFAKAGKKVTVIDILD-RPLGVYL-DKEFTDV------------------------------LTEEMEANNITIATGETVERYEGDGRVQKVVTDKNAYDADLVVVAVGVRPNTAWLKGTLELHPNGLIKTDEYMRTSEPDVFAVGDATLIKYNPADTEVNIALATNARKQGRFAVKNLEE-

General information: TITO was launched using: RESULT: Template: 1NHP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1510 32984 21.84 113.74 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 21.84 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.485

(partial model without unconserved sides chains): PDB file : Tito_1NHP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1NHP-query.scw PDB file : Tito_Scwrl_1NHP.pdb: