Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIGFFDSGIGGMTVLYEAIKVLPYEDYIFYADTLNVPYGEKSKGKVKEYIFNAAEFLASQNIKALVIACNTATSIAIEDLRRNFDFPIIG-IEPAVKPAINKCTEERKRVLVVATNLTLKEEKFHNLVKEIDHHD---LVDCLALPGLVEFAENFDFSEDKIIKYLKNELSSFDLKQYGTIVLGCTHFPFFKNSFEKLFGIKVDMISGSVGTAKQLKKVLADRNQLGKGSGSITFFNSGHKIVDQEVISKYKRLFEILDETQRSHVGH 3UHO Chain:A ((25-237)) MKIGVFDSGVGGLSVLKSLYEARLFDEIIYYGDTARVPYGVKDKDTIIKFCLEALDFFEQFQIDMLIIACNTASAYALDALRAKAHFPVYGVIDAGVEATIKALHDKNKEILVIATKATIKSEEYQKRLLSQGYTNINALATGLFVPMVEEGIFEGDFLQSAMEYYFKN-ITTPD-----ALILACTHFPLLGRSLSKYFGDKTKLIHSGDAIVEFLKE--------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3UHO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1193 -144738 -121.32 -692.53 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -121.32 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.551

(partial model without unconserved sides chains): PDB file : Tito_3UHO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3UHO-query.scw PDB file : Tito_Scwrl_3UHO.pdb: