Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYAIIKTGGKQIKVEEGQTVYIEKLAAEAGETVTFEDVLFVG-GDNVKVGNPTVEGATVTAKVEKQGRAKKITVFRYKPKKNVHKKQGHRQPYTKVTIEKINA 5ADY Chain:R ((1-103)) MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANGEEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGISA

General information: TITO was launched using: RESULT: Template: 5ADY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain R - contact count / total energy / energy per contact / energy per residue : 397 645 1.62 6.32 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain R : 0.88 3D Compatibility (PKB) : 1.62 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.88 QMean score : 0.474

(partial model without unconserved sides chains): PDB file : Tito_5ADY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5ADY-query.scw PDB file : Tito_Scwrl_5ADY.pdb: