Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTHIYKKLGAAFFALLLIAALAACGNNSESKGSASDSKGAETFTYKAENGNVKIPKHPKRVVVMADGYYGYFKTLGIN--VVGAPENVFKN-PYYKGKTNGVENIGDGTSV--EKVIDLNPDLIIVWTTQGADIKKLEKIAPTVAVKYDKLDNIEQLKEF-------AKMTGTEDKAEKWLAKWDKKVAAAKTKIKKAVGDKTISIMQTNGKDIYVFGKDFGRGGSIIYKDLGLQATKLTKEKAIDQGPGYTSISLEKLPDFAGDYIFAGPWQ----SGGDDGGVFESSIWKNLNAVKNGHVYKMDPIGFYFTDPISLEGQLEFITESLTK 3ZKW Chain:A ((27-304)) ----------------------------------SMSDEGDSFLVKDSLGENKIPKNPSKVVILDLGILDTFDALKLNDKVVGVPA---KNLPKYLQQFKNKPSVGGVQQVDFEAINALKPDLIIISGRQSKFYDKLKEIAPTLFV---GLDNANFLSSFENNVLSVAKLYGLEKEALEKISDIKNEIEKAKSIVDE---DKKALIILTNSNKISAFGPQSRFG--IIHDVLGINAV----DENIKVGTHGKSINSEFILEKNPDYIFVVDRNVILGNKERAQGILDNALVAKTKAAQNKKIIYLDPEYWYLASGNGLESLKTMILE----

General information: TITO was launched using: RESULT: Template: 3ZKW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1283 66199 51.60 252.67 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : 51.60 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.463

(partial model without unconserved sides chains): PDB file : Tito_3ZKW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ZKW-query.scw PDB file : Tito_Scwrl_3ZKW.pdb: