Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------------MKLFGMIFLIATVAFILLGVLLKLAAFFFVSILTLIAAIVLFTVLKKNQHNQT 2JZ2 Chain:A ((1-66)) MIFPGATVRVTNVDDTYYRFEGLVQRVSDGKAAVLFENGNWDKLVTFRLSELEAVKPILEHHHHHH-

General information: TITO was launched using: RESULT: Template: 2JZ2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 188 -19784 -105.23 -380.45 target 2D structure prediction score : 0.10 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -105.23 2D Compatibility (Sec. Struct. Predict.) : 0.10 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.420

(partial model without unconserved sides chains): PDB file : Tito_2JZ2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2JZ2-query.scw PDB file : Tito_Scwrl_2JZ2.pdb: