Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----MESHIYRIIKNKLTIIIFTI-----IILI----PCVDISLLLMTNTEYHPAYAFFLSGTSVGHASQMILLWFLPLYFLLLCADD---SIQDYKTGYHYILISKVGR--KKYCLEK------IFTSFIISFLTMFLSLILN----FLLVQVFFFKGTFKNDLDQIKFPDNSLYTFSMAHPYI---AIVLFSIICCIMSGFVGALGSSLSLLFRDKKYAYPASFFI---WFVLI-----------LKNKSLMFLFQPFTEYGYNVLLPILCLSIFIF-LIIISSIVLYEAKYNEN------------------ 1SVB Chain:? ((1-302)) SRCTHLENRDFVTGTQGTTRVTLVLELGGCVTITAEGKPSMDVWLDAIYQENPAKTREYCLHAKLSDTKVAA------------RCPTMGPATLAEEHQGGTVCKRDQSDRGWGNHCGLFGKGSIVACVKAACEAKKKATGHVYDANKIVYTVKVEPHTGDYVAANETHSGRKTASFTISSEKTILTMGEYGDVSLLCRVASGV--DLAQTVILELDKTVEHLPTAWQVHRDWFNDLALPWKHEGAQNWNNAERLVEFGAPHAVKMDVYNLGDQTGVLLKALAGVPVAHIEGTKYHLKSGHVTCEVGLEKLKMKGL

General information: TITO was launched using: RESULT: Template: 1SVB.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 992 -41258 -41.59 -174.08 target 2D structure prediction score : 0.33 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -41.59 2D Compatibility (Sec. Struct. Predict.) : 0.33 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.197

(partial model without unconserved sides chains): PDB file : Tito_1SVB.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1SVB-query.scw PDB file : Tito_Scwrl_1SVB.pdb: