Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLISWNVNGLRAVMRKMDFLSYLKEEDADIICLQETKIQDGQV--DLQ--PEDYHVYWNYAV-KKGYSGTAVFSKQEPLQVIYGIGVEEHDQEGRVITLEFENVFVMTVYTPNSRRGLERIDYRMQWEEALLSYILELDQKKPVILCGDLNVAHQEIDLKNPKANRNNAGFSDQEREAFTRFLEA-GFVDSFRHVYPDLEGAYSWWSYRAGARDRNIGWRIDYFVVSESLKEQIEDASISADVMGSDHCPVELIINI 5DG0 Chain:A ((20-276)) LKICSWNVDGLRAWIKKKG-LDWVKEEAPDILCLQETKCSENKLPAELQELPGLSHQYWSAPSDKEGYSGVGLLSRQCPLKVSYGIGDEEHDQEGRVIVAEFDSFVLVTAYVPNAGRGLVRLEYRQRWDEAFRKFLKGLASRKPLVLCGDLNVAHEEIDLRNPKGNKKNAGFTPQERQGFGELLQAVPLADSFRHLYPNTPYAYTFWTYMMNARSKNVGWRLDYFLLSHSLLPALCDSKIRSKALGSDHCPITLYLAL

General information: TITO was launched using: RESULT: Template: 5DG0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1357 10697 7.88 42.62 target 2D structure prediction score : 0.78 Monomeric hydrophicity matching model chain A : 0.87 3D Compatibility (PKB) : 7.88 2D Compatibility (Sec. Struct. Predict.) : 0.78 1D Compatibility (Hydrophobicity) : 0.87 QMean score : 0.604

(partial model without unconserved sides chains): PDB file : Tito_5DG0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5DG0-query.scw PDB file : Tito_Scwrl_5DG0.pdb: