Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAKGLGKGINALFNQVDLSEETVEEIKIADLRPNPYQPRKHFDDEALAELKESVLQHGILQPLIVRKSLKGYDIVAGERRFRAAKLAGLDTVPAIVRELSEALMREIALLENLQREDLSPLEEAQAYDSLLKHLDLTQEQLAKRLGKSRPHIANHLRLLTLPENIQQLIAEGTLSMGHGRTLLGLKNKNKLEPLVQKVIAEQLNVRQLEQLIQQLNQNVPRETKKKEPVKDAVLKERESYLQNYFGTTVNIKRQKKKGKIEIEFFSNEDLDRILELLSERES 4UMK Chain:C ((35-227)) ------------------------ELGIDEVMPNPYQPRKVFSEDSLEELAQSIKEHGLLQPVLVVSENGRYHLIAGERRLRASKLAKMPTIKAIVVDIEQEKMREVALIENIQREDLNPLELARSYKELLESYQMTQEELSKIVKKSRAHVANIMRLLTLSSKVQNALLEEKITSGHAKVLVGLDGE-KQELILNSIIGQKLSVRQTEDLARDFKIN----------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4UMK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 636 -50784 -79.85 -263.13 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain C : 0.77 3D Compatibility (PKB) : -79.85 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.530

(partial model without unconserved sides chains): PDB file : Tito_4UMK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4UMK-query.scw PDB file : Tito_Scwrl_4UMK.pdb: