Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLDTQQIKEIIPHRYPFLLVDRITEVEEGKRAKGYKNVTANEEFFNGHFPQYPVMPGVLIVEALAQVGAVAMLIKEE------NRGRLAFFAGIDNCRFKKQVKPGDQLHLEVEIIRARGTIGRGKGVATVDGEVVCEVELTFALGE 2GLL Chain:A ((24-168)) -FFIEHILQILPHRYPMLLVDRITELQANQKIVAYKNITFNEDVFNGHFPNKPIFPGVLIVEGMAQSGGFLAFTSLWGFDPEIAKTKIVYFMTIDKVKFRIPVTPGDRLEYHLEVLKHKGMIWQVGGTAQVDGKVVAEAELKAMIA-

General information: TITO was launched using: RESULT: Template: 2GLL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 650 -57908 -89.09 -416.60 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.83 3D Compatibility (PKB) : -89.09 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.573

(partial model without unconserved sides chains): PDB file : Tito_2GLL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2GLL-query.scw PDB file : Tito_Scwrl_2GLL.pdb: