Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDIRQLRYFITIAQEQKITSAAKKLHMAQPPLSRQLKQLEDELGVVLFDRNKKKQMTLTYEGAVFLKRAKEILHRFEDAVIEVQELKEEVAGTLAVGSTIYCAALMLEKVTQIKER-YPHLTFNIWENEPATLLELLESRQIDAAVTTTL---IKSD-TVQFKQLDDIPCVLVLSDEAGYPCGDTIKMVDIPSFPLILLRPVNGKGVYNQVMNEFHRLNL-EPRIVCECHDSATLLSLVSSGFGASILPVTMIPVHMRNHVHTVHIENNPFIMTPAVMWRTDSYLPKPAQQFLDLF 3FXU Chain:A ((2-246)) LKLQTLQALICIEEVGSLRAAAQLLHLSQPALSAAIQQLEDELKAPLLVRTKRG-VSLTSFGQAFMKHARLIVTESRRAQEEIGQLRGRWEGHITFAASPAIALAALPLALASFAREFPDVTVNVRDGMYPAVSPQLRDGTLDFALTAAHKHDIDTDLEAQPLYVSDV----VIVGQRQHPMANATRLAELQECRWAFSSAPRGPGAI--IRNAFARYGLPEPKLGLVCESFLALPGVVAHSDLLTTMPRTL--------------------------------------------

General information: TITO was launched using: RESULT: Template: 3FXU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 874 -134055 -153.38 -560.90 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -153.38 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.504

(partial model without unconserved sides chains): PDB file : Tito_3FXU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3FXU-query.scw PDB file : Tito_Scwrl_3FXU.pdb: