Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKVLKPGLLTTVQDIGRTGYQKYGVLASGAMDTVSLRIANLLIGNGENEAGLEITMMGPGPSFHFSKQTLIAVTGADFTLRINDEEAPLWKPVLIKENSTVSFGPCKLGSRAYLAAAGGIEVPAVMESKSTYVRGSIGGLHGRALQKEDELNIGEMSALSQTILSRLSSQLGKQGFAAPKWSVSRGRFLPLKKNPVIRVLEGKQFAFFTEESKTRFYEEAFRVTPQSDRMGYRLKGE---PLELKAPLEMVSEAVSFGTVQVPPDGNPIILLADRQTTGGYPRIAHIISADLPIVS-----QIMPGEHVQFEPVSLQEAEALAVEREQHIKELKTRMKMEWLT 2PHC Chain:B ((112-217)) -----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DDVIEIHSKPLYRVYFLGFLPGFAYLGGMDERIATP-RLEKPRLKVPAGSVGIAGK-QTGWYAIES--PGGWRIIGRI-PLRTFNPGKVPPSIVLPGDYVKFVPIDEKEFW-----------------------

General information: TITO was launched using: RESULT: Template: 2PHC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 410 -41432 -101.05 -422.78 target 2D structure prediction score : 0.42 Monomeric hydrophicity matching model chain B : 0.54 3D Compatibility (PKB) : -101.05 2D Compatibility (Sec. Struct. Predict.) : 0.42 1D Compatibility (Hydrophobicity) : 0.54 QMean score : 0.390

(partial model without unconserved sides chains): PDB file : Tito_2PHC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2PHC-query.scw PDB file : Tito_Scwrl_2PHC.pdb: