Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEIAFLNSLVV---TSPGFYKAEKITLDEV--KQWLKHYDGRYKSFIGHKSTAQFLQKLLGIRIE--------QNRKTFRHMKYQKAICFSLY-----ERYPEN-VLLTQRDLEKARYQFYLLTRLD 1SSF Chain:A ((7-129)) --NSFVGLRVVAKWSSNGYFYSGKITRDVGAGKYKLLFDDGYECDVLG-KDILLCDPIPLDTEVTALSEDEYFSAGVVKGHRKESGELYYSIEKEGQRKWYKRMAVILSLEQGNRLREQ-YGLGPYE

General information: TITO was launched using: RESULT: Template: 1SSF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 506 -36149 -71.44 -347.59 target 2D structure prediction score : 0.26 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -71.44 2D Compatibility (Sec. Struct. Predict.) : 0.26 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.169

(partial model without unconserved sides chains): PDB file : Tito_1SSF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1SSF-query.scw PDB file : Tito_Scwrl_1SSF.pdb: