Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDVRNKTLWILRDYVYILIGAAITAVSFNVFLLPNKIAAGGVSGISTILQSYGFEAAYVQWIINIPLFIAGVILLGGKFGLKTLAGSVFLPLVVFLTRDIQPATHHELLAAIFGGVGIGIGIGIVYLGKGSTGGTALAAQIIHKYSGLSLGKCLAIIDGMIVVTAMIVFNIEQGLYAMLGVYVSSKTIDVVQVGFNRSKMALIITKQ--EQAVKEAVLQKIDRGVTKISAVG--GYTD---------DDRPILMCVVGQTEFTKLKQIVKQIDE-----SAFVIVADASEVLGEGFKRA 4AFF Chain:A ((3-101)) -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KIEAIIRPFKLDEVKIALVNAGIVGMTVSEVRGFGRQK-RGSEYTVEFLQKLKLEIVVEDAQVDTVIDKIVAAARTGENGDGKIFVSPVDQTIR------

General information: TITO was launched using: RESULT: Template: 4AFF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 241 11723 48.64 156.31 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.44 3D Compatibility (PKB) : 48.64 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.44 QMean score : 0.592

(partial model without unconserved sides chains): PDB file : Tito_4AFF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4AFF-query.scw PDB file : Tito_Scwrl_4AFF.pdb: