Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKVIFLQDVKGKGKKGEVKNVADGYAHNFLIKKGLAVEANASNISALNGQKQKEKKEAIAELEQAKSLKETLEKL-TVELSAKSGEGGRLFGSVTSKQITEQLQKDHNIKVDKRKLELPDGI-RALGYTNVPVKLHPEVQAVLKVHVKEEA 5GAE Chain:H ((1-149)) MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNIEFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADAVTA-AGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE-

General information: TITO was launched using: RESULT: Template: 5GAE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain H - contact count / total energy / energy per contact / energy per residue : 563 -18237 -32.39 -124.06 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain H : 0.79 3D Compatibility (PKB) : -32.39 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.461

(partial model without unconserved sides chains): PDB file : Tito_5GAE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5GAE-query.scw PDB file : Tito_Scwrl_5GAE.pdb: