Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAIQLSKGQRIDLTKTNPGLTKAVIGLGWDTNKY-SGGHDFDLDASAFLVDAHDNCVNDLDFVFYNNLEHPSGGVIHTGDNRTGEGDGDDEQI-IVDFSKIPAHIEKIGITVTIHDAEARSQNFGQVSNAFV-RVVDEETQNELLRFDLGEDFSIETAVVVCELYRHG-GEWKFNAIGSGFSGGLAALCRN-YGLQV 2QNG Chain:A ((28-197)) -----------------KGIRKVELAVKWDPSPPGDPATDLDIVAATFLAGDAYG---KPAYVVHFDSRSPDGTIYLNRDSKDGKGFGWDE-VMTLELNRLDSRYARVVVGVVIQQRDAHRTFVGVLNPGLRMRE----GYTVLAEDDFGGVLG-STAATVGEFVRDDSGEWTFHPGIHGYDSDPATFARVMGGRQ-

General information: TITO was launched using: RESULT: Template: 2QNG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 889 -65113 -73.24 -394.62 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -73.24 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.362

(partial model without unconserved sides chains): PDB file : Tito_2QNG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2QNG-query.scw PDB file : Tito_Scwrl_2QNG.pdb: