Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTFFQFDELGTSYRNEIIGGLTTFLSMAYILFVNPITLALESVKDFPEALRIDQGAVFTATALASAAGCILMGLI-A-RYPIAIAPGMGLNAFFAFSVVLGMGISWQAALSGVFISGLIFVALSLT----GFREKIINAIPPELKLAVGAGIGLFITFVGLQGSGIITANPSTLVTIGNIHSGPVLLTIFGVIVTVILMVL-----RVNAGVFIGMLLTAVAGMIFGLVPVP----TQIIGSVPSLAPTFGQAWIHLPDIFSVQMLIVILTFLFVGFFDTAGTLVAVATQAGLMKENKLPRAGRALLADSSSIVIGAVLGTSTTTSY-VESSSGVAAGARSGFAAIVTGILFLLATF---FSPLLSVVTSNVTAPALIIVGALMVAPLGKIAWDKFEVAVPAFLTMIMMPLTYSIATGIAIGFIFYPITMVCKGKAKEVHPIMYGLFVVFILYFIFLK 3QE7 Chain:A ((4-341)) --RAIGVSERPP-LLQTIPLSLQHLFAMFGATVLVPVLFH----------------INPATVLLFNGIGTLLYLFICKGKIPAYLGSSFAF-ISPVLLLL---PLGYEVALGGFIMCGVLFCLVSFIVKKAGT-GWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAE------GQTPDSKTIIISITTLAVTVLGSVLFRGFLAIIPIL-IGVLVGYALSFAMGIVDTTPIINAHWFAL-----PTLYT--PRFE----WFAILTILPAALVVIAEHVGHLVVTANIVKK-DLLRDPGLHRSMFANGLSTVISGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVMGGVSLLL------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3QE7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1550 -238618 -153.95 -748.02 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -153.95 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.290

(partial model without unconserved sides chains): PDB file : Tito_3QE7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3QE7-query.scw PDB file : Tito_Scwrl_3QE7.pdb: