Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNDFSLELPVRTNKPRETGQSILIDNGYPLQFFKDAIAGASDYIDFVKFGWGTSLL--TKDLEEKISTLKEH-DITFFFGGTLFEKYVSQKKVNEF-HRYCTYFGCEYIEISNGTLPMTNKEKAAYIADFSD-EFLVLSEVGSKDAELASRQSSEEWLEYIVEDMEAGAEKVITEARESGTGGICSSSGDVRFQIVDDIISSDIDINRLIFEAPNKTLQQGFIQKIGPNVNLANIPFHDAIALETLRLGLRSDTFFL 3F4W Chain:A ((11-138)) ----------------------------TLPEAMVFMDKVVDDVDIIEVGTPFLIREGVNAIKAIK---EKYPHKEVLADAKIMD-------GGHFESQLLFDAGADYVTVLGVTDVL---TIQSCIRAAKEAGKQVVVDMICVD-------DLP---ARVRLLEEAGADMLAVHTGTD------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3F4W.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 545 -59978 -110.05 -487.63 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : -110.05 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.372

(partial model without unconserved sides chains): PDB file : Tito_3F4W.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3F4W-query.scw PDB file : Tito_Scwrl_3F4W.pdb: