Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKYGIVLFPSKKLQDLANSYRKRYD--PSYSLIPP---HLTLRASFECAEEKADQLVSHLRNIAKESHPLVLKMTKYSSFAP--VNNVIYIKAEPTEELKTLNEKLYTGVLA--------G-EQEYNFVPHVTVGQNLSDDEHSDVLGQLKMQEVSHEEIVDRFHLLYQL-E--NGSWTVYETFLLGRGE 1IUH Chain:A ((1-182)) MRLFYAVFLPEEVRAALVEAQTKVRPFRGWKPVPPHQLHLTLLFLGERPEEELPDYLALGHRLARLEAPFRARLRGTGYFPNEGTPRVWFAKAEA-EGFLRLAEGLRAGVEELLGEEAVRIPGWDKPFKPHITLARRKAPAPRVP------PVLFGLEWPVEGFALVRSELKPKGPVYTVLEKFSLRGE-

General information: TITO was launched using: RESULT: Template: 1IUH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 758 -98638 -130.13 -605.14 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -130.13 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.465

(partial model without unconserved sides chains): PDB file : Tito_1IUH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1IUH-query.scw PDB file : Tito_Scwrl_1IUH.pdb: