Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTTRKPFIICDFDGTITMNDNIINIMKTFAPPE-WMALKDGVLSKTLSIKEGVGRMFGLLPSSLKEEITSFVLEDAKIREGFREFVAFINEHEIPFYVISGGMDFFVYPLLEGIVEKDRIYCNHASFDNDYIHIDWPHSCKGTCSNQCGCCKPSVIHELSEPNQYIIMIGDSVTDVEAAKLSDLCFARDYLLNECREQNLNHLPYQDFYEIRKEIENVKEVQEWLQNKNAGESSLK 3M1Y Chain:A ((2-184)) --SLQKLAVFDFDSTLVNAETIESLARAWGVFDEVKTITLK----ETDFHKSLILRVSKLKNMPLKLAKEVCE-SLPLFEGALELVSALKEKNYKVVCFSGGFDLATNHYRDLLHL-DAAFSNTLIVENDALNGLVTG-HMMFSH-SKGEMLLVLQRLLNISKTNTLVVGDGANDLSMFKHAHIKIAFN-----------------------------------------------

General information: TITO was launched using: RESULT: Template: 3M1Y.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 916 -125614 -137.13 -705.70 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -137.13 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.329

(partial model without unconserved sides chains): PDB file : Tito_3M1Y.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3M1Y-query.scw PDB file : Tito_Scwrl_3M1Y.pdb: