Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIAKVGNVIEFKN----GLTGVVEKVN-ENSVIVDVTIMDNYRDLELDSLTVVNHKNYKIIRDSY 5GAE Chain:V ((3-37)) -AKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVE------------------------------

General information: TITO was launched using: RESULT: Template: 5GAE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain V - contact count / total energy / energy per contact / energy per residue : 72 -9086 -126.19 -302.85 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain V : 0.56 3D Compatibility (PKB) : -126.19 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.828

(partial model without unconserved sides chains): PDB file : Tito_5GAE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5GAE-query.scw PDB file : Tito_Scwrl_5GAE.pdb: