Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSQLMGIITRLQSLQETAEAANEPMQRYFEVNGEKICSVKYFEKNQTFELTVFQKGEKPNTYPFDNIDMVSIEIFELLQ 4CHM Chain:A ((35-66)) ---------------------------------RLQCILHYNEADSSLSISCED---KVRVIPLSDIK-----------

General information: TITO was launched using: RESULT: Template: 4CHM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 79 -7596 -96.15 -237.36 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -96.15 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.366

(partial model without unconserved sides chains): PDB file : Tito_4CHM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4CHM-query.scw PDB file : Tito_Scwrl_4CHM.pdb: