Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRLSELSGKEIVDIKRAERLGVLGQTDLEINEQDGQITALLIPTVKWF---GLRKQGHDIRVPWHHIQKIGSDMIILDVPEEMPPRQE 1YFN Chain:A ((45-94)) ---------------------YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIY-----------------

General information: TITO was launched using: RESULT: Template: 1YFN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 90 -18985 -210.94 -403.94 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -210.94 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.332

(partial model without unconserved sides chains): PDB file : Tito_1YFN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1YFN-query.scw PDB file : Tito_Scwrl_1YFN.pdb: