Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLKKWLAGILLIMLVGYTGWNLYQTYSKKEVGIQEGQQAPDFSLKTLSGEKSSLQDAKGKKVLLNFWATWCKPCRQEMPAMEKLQKEYADKLAVVAVNFTSAEKSEKQVRAFADTYD----LTFPILIDKKG-INADYNVMSYPTTYILDEKGVIQDIHVGTMTKKEMEQKLDLD 4EVM Chain:A ((51-184)) -------------------------------------EVADFELMGVDGKTYRLSDYKGKKVYLKFWASWCSICLASLPDTDEIAKEAGDDYVVLTV-VSPGHKGEQSEADFKNWYKGLDYKNLPVLVDPSGKLLETYGVRSYPTQAFIDKEGKLVKTHPGFMEKDAILQTL---

General information: TITO was launched using: RESULT: Template: 4EVM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 578 -18876 -32.66 -146.32 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -32.66 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.667

(partial model without unconserved sides chains): PDB file : Tito_4EVM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4EVM-query.scw PDB file : Tito_Scwrl_4EVM.pdb: