Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKVIKGLTAGLIFLFLCACGGQQIKDPLNYEVEPFTFQNQDGKNVSLESLKGEVWLADFIFTNCETICPPMTAHMTDLQKKLKAENIDVRIISFSVDPENDKPKQLKKFAANYPLSFDNWDFLTGYSQSEIEEFALKSFKAI-VKKPEGEDQVIHQSSFYLVGPDGKVLKDYNGVENTPYDDIISDVKSASTLK 3ME8 Chain:A ((12-161)) -----------------------------------TLVDSYGNEFQLKNLKGKPIILSPIYTHCRAACPLITKSLLKVIPKLGTPGKDFWVITFTFDPK-DTLEDIKRFQKEYGIDGKGWKVV----KAKTSEDLFKLLDAIDFRFMTAGNDFIHPNVVVVLSPELQI-KDYIYGVNYNYLEFVNALRLAR---

General information: TITO was launched using: RESULT: Template: 3ME8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 700 -61428 -87.75 -412.27 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -87.75 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.454

(partial model without unconserved sides chains): PDB file : Tito_3ME8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ME8-query.scw PDB file : Tito_Scwrl_3ME8.pdb: