Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKSLEEKTIAKEQIFSGKVIDLYVEDVELPNGKASKREIVKHPGAVAVLAVTDEGKIIMVKQFRKPLERTIVEIPAGKLEKGEEPEYTALRELEEETGYTAKKLTKITAFYTSPGFADEIVHVFLAEELSVLEEKRELDEDEFVEVMEVTLEDALKLVESREVYDAKTAYAIQYLQLKEALQAQK 1V8V Chain:A ((11-150)) ----------RTYLYRGRILNLALE---------GRYEIVEHKPAVAVIALR-EGRMLFVRQMRPAVGLAPLEIPAGLIEPGEDPLEAARRELAEQTGLSG-DLTYLFSYFVSPGFTDEKTHVFLAENLKEVEA-------EAIEVVWMRPEEALERHQRGEV----------------------

General information: TITO was launched using: RESULT: Template: 1V8V.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 530 -54583 -102.99 -404.32 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -102.99 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.409

(partial model without unconserved sides chains): PDB file : Tito_1V8V.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1V8V-query.scw PDB file : Tito_Scwrl_1V8V.pdb: