Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDPSTLMILAIVAVAIIVLAVFFTFVPVMLWISALAAGVKISIFTLVGMRLRRVI-PNRVVNPLIKAHKAGLNVGTNQLESHYLAGGNVDRVVNALIAAQRA------NIE--LTFERCAAIDLAGRDVLEAVQMSVNPKVIETPFIAGVAMDGIEVKAKA-RITVRANIERLVGGAGEETIVARVGEGIVSTIGSSDNHKK---VLENPDMIS-QTVLGKGLDSGTAFEILSIDIADVDIGKNIGAILQTDQAEADKNIA-QAKAEERRAMAVAQEQEMRARVEEMRAKVVEAEAEVPLAMAEALREGNIGVMDYMNIKNIDADTEMRDSFGKLTKDPSDEDRKS 1HN9 Chain:A ((33-293)) ------------------------------------------IVTRTGIRERHIAAPNETVSTM------GFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKGCPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDGAGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVTELAHI-VDETLAAN-NLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTSAASVPCALDEAVRDGRI-----------------------------------

General information: TITO was launched using: RESULT: Template: 1HN9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1258 -4291 -3.41 -17.44 target 2D structure prediction score : 0.31 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -3.41 2D Compatibility (Sec. Struct. Predict.) : 0.31 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.091

(partial model without unconserved sides chains): PDB file : Tito_1HN9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1HN9-query.scw PDB file : Tito_Scwrl_1HN9.pdb: