Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------------------------------------------------------------------MMTGTLGTLVP---IILMFAVLYFLLIRPQQKQQKAVRQMQEELKKGDSVVTIGGLHGTVDSIDESKVVIKTGDNTRLTFDRRAIREVSAAE------------------------------------------------------------------------------------------------------- 4MB0 Chain:A ((9-261)) PRYWSLYYREKIIEGMEKGMTAKAGLIAHGRGEAFDYLIGERTIEPAERAMRAAVAKLLLAENPVVSVNGNVAALVPKETIELARALNAKLEINLFYRTEDRVKAIAEELRKYDPEIELLGIN--------PTKRIPGLEHERGKVDENGIWKADVVVVPLEDGDRTEALVRMGKFVITIDLNPLSRSARMADITIVDNIVRAYPRMTELAREMKDYSRGELIRIIEEYDNGKTLNDVLLHIRDRLTKLAEGGIWRKKQLD

General information: TITO was launched using: RESULT: Template: 4MB0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 159 440 2.77 5.43 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : 2.77 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.448

(partial model without unconserved sides chains): PDB file : Tito_4MB0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4MB0-query.scw PDB file : Tito_Scwrl_4MB0.pdb: