Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPKMKTHRGSAKRFKKTGSGKLKRSHAYTSHLFANKSQKQKRKLRKSA--VVSAGDFKRIKQMLANIK 3K4S Chain:A ((184-220)) -------------------------------IFQNLTKKQRQSLRKMVIDIVLATDMSKHMNLLADLK

General information: TITO was launched using: RESULT: Template: 3K4S.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 26 2861 110.02 81.73 target 2D structure prediction score : 0.80 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : 110.02 2D Compatibility (Sec. Struct. Predict.) : 0.80 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.783

(partial model without unconserved sides chains): PDB file : Tito_3K4S.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3K4S-query.scw PDB file : Tito_Scwrl_3K4S.pdb: