Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTDQRRNVYFFHKQDQETNEQARSLTQLAEEHGFTVVNQHSDANIIASIGGDGTFLQAVRKTNFR-DDCLYVGITKKGKAHLYCDFHSDEREKMVDAMTFEQIEVRKYPLIEVTVD---QASPFHCLNEVSIRSSIIKTFVMDVLIDDLHFETFRGDGMIISTPTGSTAYNKSVAGAVVDPLLPCMQVSELASLNNNTYRTLGSPFVLSSDRKLTLRVVQDGNEHPIIGLDNEALSTRNVKTIEIKLSNKKIKTVKLKDNSFWEKVKRTFL 2I2F Chain:A ((35-262)) -----------------------------------------EPEIVISIGGNGTFLSAFHQYEERLDEIAFIGI-HTGHLGFYADWRPAEADKLVKLLAKGEYQKVSYPLLKTTVKYGKKEATYLALNESTVKSSG-GPFVVDVVINDIHFERFRGDGLCMSTPSGTTAYNKSLGGALMHPSIEAMQLTEMASINNRVYRTIGSPLVFPKHHVVSLQPVNDKDFQ--ISVDHLSILHRDVQEIRYEVSAKKIHFARFRSFPFWRRVHDSFI

General information: TITO was launched using: RESULT: Template: 2I2F.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1162 22792 19.61 102.67 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.83 3D Compatibility (PKB) : 19.61 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.415

(partial model without unconserved sides chains): PDB file : Tito_2I2F.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2I2F-query.scw PDB file : Tito_Scwrl_2I2F.pdb: