Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNGRTLFFRRLFDYYKYQFKVLHAVIDWTVALYIVLPAIAFVIYQYIDLMNGRGLLYEWSEVAEWRWLYAVCVLIMFTGSIRTFLMEADKVFLLQKKEIIYQLKRYALLYSFLATLAKWLLLFFIVLPLISHSVLITFAESTALLCYLFGLHIFFLSLKQDRIRKPRSISRWIGDTLVRAILFAGSAILIVFTERHLLALFGILFLFFSVIRSLKKTASFTAFEAEVTEEKKSRLALAGLVMMMSQEAGMPKVKDRMRRKPLLYRNSKRIFKRRTICTGYKELFFKVMLRNGEYARQMYMLLSAFTVLIFVSPIWLKVIALLVYTGVCRYILTLIFDKVMDAPFLIGTDKESDEYYRARKSCINILHYAFAACCFLAAAVSLLFT 2F95 Chain:B ((24-76)) ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------IFVGALTVLFGAIAYGEVTAAAATGDAAAVQEAAVSAILGLIILLGINLGLVA--------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2F95.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 80 -3688 -46.09 -69.58 target 2D structure prediction score : 0.83 Monomeric hydrophicity matching model chain B : 0.43 3D Compatibility (PKB) : -46.09 2D Compatibility (Sec. Struct. Predict.) : 0.83 1D Compatibility (Hydrophobicity) : 0.43 QMean score : 0.263

(partial model without unconserved sides chains): PDB file : Tito_2F95.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2F95-query.scw PDB file : Tito_Scwrl_2F95.pdb: