Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MWKRMTAKAEGL---------YIADTKSFVTKQMDKLDFDYGGIPGDLHFGLTKKAGAREPMFSRGT--------EIFNRRQISIVSIEECNEIALKMGVPRILPEWLGANVAVSGMPDLTSLK----EGSRIIFPSGAALLCEGENDPCIQPGEVIQSYYPDQPKLASAFVRHALGIRGIVCIVERPGAVYTGDEIEVHSYQRKVKRKAERV 4YCZ Chain:C ((968-1224)) MLSRLFTAATSISHFEEAHSALLSMMQKSYLRRLVEKMCETGQSSELITLPFSGLQTKVDDILVEKCRATRVPYHQILYAWRINHNDYRGGAAILLDRLQKLRRAGALDTQVTRQYLLLINALSCVPPQEAYILEDDDLEADIDELEKKLDVEGEDAALIEKMKRFSTR----------------NGARRLLMLADLR-KQYQQELDRIVAI-

General information: TITO was launched using: RESULT: Template: 4YCZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 430 10771 25.05 61.90 target 2D structure prediction score : 0.45 Monomeric hydrophicity matching model chain C : 0.61 3D Compatibility (PKB) : 25.05 2D Compatibility (Sec. Struct. Predict.) : 0.45 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.039

(partial model without unconserved sides chains): PDB file : Tito_4YCZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4YCZ-query.scw PDB file : Tito_Scwrl_4YCZ.pdb: