Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------------MPSITKQIMSGAKWTSISTMCITIIQIVQFALLGNMMTLTEFGLVGMITTVTVFAQIVLDMG-FGAALIQRDDATERQLSTLYWLNIMTGVLLFVLLYVSSPVIAGFYQREELVFLVRILAIMFLIAPIGQQYQYMLQKQLH---------FNTLSKIEIFSNVLSFGYLAIAVFMMDAILAYVISQVLL--------QSSKGILYWAVYRKKWHPAFVFD------LRGMKDFF------------SFGAFQLSSRLVNRLGANI--DMILIGRFIGAEALGIYN-LAYQIVTIPVLKINPIVTRVAFPI--FAKNKYENSVIREGFLNMTKMLAL--VSFPLLIGLV--SVSDAFITAVFGEKWLAAVPILNVLAIVGILRVLMNPNGSVLLAKGRADLAFYWD--SGVLLLYGLSLFAAVQTG-SLLTVAWVYAIISVVNFLIGRWLLAYV-----IKLNLSAYFQSIMKPFLITAAMGIIAFGVSLSTEHFSMQAEMRLAISVAAGALCYLFLLVKAYPQTKSKLLRKGRLS 3EPO Chain:A ((5-546)) STIKAVAETISTGPIPGSRKVYQAGELFPEL---RVPFREVAVHPSANEP-PVTIYDPSGPYSDPAIQIDIEKGLPRTREALVVARGDVEEVADPRQVPEFPDTGRKIYRAKPGKLVTQLEYARAGI-----ITAEMEYVAIRENLRREQDRPCVRDGEDFGASIPDFVTPEFVRQEIARGRAIIPANINHGELEPMAIGRNFLVKINANIGNTVADEVDKLVWATRWGADTVMDLSTGRNIHNIRDWIIRNSSVPIGTVPIYQALEKVNGVAEDLNWEVFRDTLIEQCEQGVDYFTIHAGVRLPFIPMTAKRVTGIVSRGGSIMAKWCLAHHKENFLYERFDEICEIMRAYDVSFSLGDGLRPGSTADANDEAQFSE--LRTLGELTKVAWKHGVQVMIEGPGHVAMHKIKANMDEQLKHCHEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWF----GTAMLCYVTPKEHLGLPDRDDVKTGVITYKLAAHAADLAKGHPGAAMWDDAISRARFEFRWEDQFN------LGLDPETARKFH------

General information: TITO was launched using: RESULT: Template: 3EPO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2719 -124941 -45.95 -273.99 target 2D structure prediction score : 0.37 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -45.95 2D Compatibility (Sec. Struct. Predict.) : 0.37 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.248

(partial model without unconserved sides chains): PDB file : Tito_3EPO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3EPO-query.scw PDB file : Tito_Scwrl_3EPO.pdb: