Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----MKALIPKQHG-------AWAMLLIPFLLG----MVKGGPVIWHIPL----FLGWLFLYLAVYPVTLALKKKQSKPYQKWMCYYG----FPTCCFLMISVFHK--PPLIWVGVSLLP-LFLIHMYFA--------RRKNERALLNDVAGVLF----FCSGGFASCWLGMGTLDGWAWFIFLQSALFFI-GSSFYVKSV-IRERKNRAFAYWSW--GYHLLL--------------PFLSALFGAGWAFL----AFIPSSLRAWFFHGRDWPVKTIGILEIVNACFFLAV-----MCLFITR----- 4MUO Chain:A ((1-320)) MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGGVLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLAILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGAFCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDIGGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQAKDPETTAQWIERCLAGSEPIPESLKIQMACCLVATGEAATISDGLARVNQAF

General information: TITO was launched using: RESULT: Template: 4MUO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1233 -85055 -68.98 -355.88 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -68.98 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.258

(partial model without unconserved sides chains): PDB file : Tito_4MUO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4MUO-query.scw PDB file : Tito_Scwrl_4MUO.pdb: