Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFIEQMFPFINESVRVHQLPEGGVLEIDYLRDNVSISDFEYLDLNKTAYELCMRMDGQKTAEQILAEQCAVYDESPEDHKDWYYDMLNMLQNKQVIQLGNRASRHTITTSGSNEFPMPLHATFELTHRCNLKCAHCYLESSPE-------ALGTVSIEQFKKTADMLFDNGVLTCEITGGEIFVHPNANEILDYVCKK-FK-KVAVLTNGTLMRKESLELLKTYKQKIIVGISLDSVNSEVHDSFRGRKGSFAQTCKTIKLLSDHGIFVRVAMSVFEK-NMWEIHDMAQKVRDLGAKAFSYNWVDDFGRGRDIVH-PTKDAEQHRKFMEYEQHV---I-DEFK---DLI--PI----I-PYERKRAANCGAGWKSIVISPFGEVRPCALFPK------EFSLGNI----FHDSYESIFNSPLVHKLWQAQAPRFSEHCMKDKCPFSGYCGGCYLKGLNSNKYHRKNICSWAKNEQLEDVVQLI 1TV8 Chain:A ((12-312)) --------------------------------------------------------------------------------------------------------------------PIRDLRLSVTDRCNFRCDYCMPKEVFGDDFVFLPKNELLTFDEMARIAKVYAELGVKKIRITGGEPLMRRDLDVLIAKLNQIDGIEDIGLTTNGLLLKKH-GQKLYDAG-LRRINVSLDAIDDTLFQSINNRNIKATTILEQIDYATSIGLNVKVNVVIQKGINDDQIIPMLEYFKDKHIE-IRFIEFMDVGNDNGWDFSKVVTKDEMLTMIEQHFEIDPVEPKYFGEVAKYYRHKDNGVQFGLITSVSQSFCSTC-TRARLSSDGKFYGCLFATVDGFNVKAFIRSGVTDEELKEQFKALWQIR--------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1TV8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1294 4986 3.85 18.74 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 3.85 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.411

(partial model without unconserved sides chains): PDB file : Tito_1TV8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1TV8-query.scw PDB file : Tito_Scwrl_1TV8.pdb: