Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTLHKERRIGRLSVLLLLNEAEESTQVEELERDGWKVCLGKVGSMDAHKVVAAIETASKKSGVIQSEGYRESHALYHATMEALHGVTRGEMLLGSLLRTVGLRFAVLRGNPYESEAEGDWIAVSLYGTIGAPIKGLEHETFGVGINHI 4H4L Chain:H ((2-148)) -TLHKERRIGRLSVLLLLNEA----QVEELERDGWKVCLGKVGSMDAHKVIAAIETASKKSGVIQSEGYRESHALYHATMEALHGVTRGEMLLGSLLRTVGLRFAVLRGNPYESEAEGDWIAVSLYGTIGAPIKGLEHETFGVGINHI

General information: TITO was launched using: RESULT: Template: 4H4L.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain H - contact count / total energy / energy per contact / energy per residue : 684 -99490 -145.45 -695.73 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain H : 0.99 3D Compatibility (PKB) : -145.45 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.99 QMean score : 0.589

(partial model without unconserved sides chains): PDB file : Tito_4H4L.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4H4L-query.scw PDB file : Tito_Scwrl_4H4L.pdb: