Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFRLLVLMGFTFFFYHLHASGNLTKYINMKYAYLSFIAIFLLAILTAVQAYLFIKSPEKSGHHHDHDCGCGHDHEHDHEQNKPFYQRYLIYVVFLFPLVSGIFFPIATLDSSIVKTKGFSFKAMESGDHYSQTQYLRPDASLYYAQDSYDKQMKQLFNKYSSKKEISLTDDDFLKGMETIYNYPGEFLGRTIEFHGFAYKGNAIN--K-NQLFVLRFGIIHCIADSGVYGMLVEFPK----DMDIKDDEWIHIKGTLASEYYQPFKS-TLPVVKVTDWNTIKKPDDPYVYRGF 3KOJ Chain:A ((3-97)) ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------SCILQATVVEAPQLRYAQDNQTPVAEMVVQFPG-APARLKVVGWGAVAQELQDRCRLNDEVVLEGRLRINS------EKQTELTVTRVH--------------

General information: TITO was launched using: RESULT: Template: 3KOJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 325 -47359 -145.72 -639.98 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.53 3D Compatibility (PKB) : -145.72 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.53 QMean score : 0.407

(partial model without unconserved sides chains): PDB file : Tito_3KOJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3KOJ-query.scw PDB file : Tito_Scwrl_3KOJ.pdb: